Protocols for Studying Pathogenic Fungi

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Springer


Paru le : 2025-05-23

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Description

This book is a compilation of protocols for conducting experiments with a variety of yeasts, including S. cerevisiae, Candida albicans, non-albicans Candida sp., Cryptococcus sp.; and molds, including Aspergillus sp., Fusarium sp., Mucor, Rhizopus. The chapters not only discuss National Committee for Clinical Laboratory Standards (NCCLS) accredited protocols but also emphasize the significance of Biosafety Level-2 (BSL-2) facilities. Subsequent chapters cover the essentials of handling, culturing, preserving, and reviving various fungal pathogens. Moreover, advanced techniques are explored, including counting colony-forming units (CFU), growth curve preparation, and microtiter plate assays. A separate chapter discusses the protocols for disc-diffusion, spot and kill-kinetics assays, addressing analyses like a checkerboard and Fractional Inhibitory Concentration (FIC) index assessments. Additionally, the book covers the formation of biofilm, capsule and melanin, fungal cell adhesion, and sterols extraction. Finally, it elaborates on the optimized impact achieved by assessing mitochondrial fitness through non-fermentable carbon.


This book is relevant for mycologists and scientists working in the field, providing insights into fungal pathogens and protocols for handling different fungal species.
Pages
262 pages
Collection
n.c
Parution
2025-05-23
Marque
Springer
EAN papier
9789819670468
EAN PDF
9789819670475

Informations sur l'ebook
Nombre pages copiables
2
Nombre pages imprimables
26
Taille du fichier
22426 Ko
Prix
168,79 €
EAN EPUB
9789819670475

Informations sur l'ebook
Nombre pages copiables
2
Nombre pages imprimables
26
Taille du fichier
14612 Ko
Prix
168,79 €

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