Téléchargez le livre : Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

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Éditeur :

Springer


Collection :

Springer Theses

Paru le : 2017-07-20

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Description

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successfuldevelopment and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.
Pages
114 pages
Collection
Springer Theses
Parution
2017-07-20
Marque
Springer
EAN papier
9789811047527
EAN PDF
9789811047534
Informations sur l'ebook
Nombre pages copiables
1
Nombre pages imprimables
11
Taille du fichier
5294 Ko
Prix
94,94 €
EAN EPUB
9789811047534
Informations sur l'ebook
Nombre pages copiables
1
Nombre pages imprimables
11
Taille du fichier
4045 Ko
Prix
94,94 €

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